Signalling

Part:BBa_K2323006:Design

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-26)


GFP-LAA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

Source

This degradation tag is taken from Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998. It can be degraded at a rate of 0.0327/min by the ClpP machinery of E.coli.

References

Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998